![Human japanese intermediate crack](https://loka.nahovitsyn.com/76.jpg)
![cellprofiler metadata cellprofiler metadata](https://i1.rgstatic.net/publication/6536157_CellProfilerTM_Free_versatile_software_for_automated_biological_image_analysis/links/0deec51b1c7e80bd23000000/largepreview.png)
Peripheral whole blood of three healthy donors was exposed to doses from 0-20 Gy, with 60Co gamma radiation. In the present work we estimated the doses and the percentage of the irradiated fraction in simulated partial-body radiation exposures at high doses using the PCC-R assay. The PCC-R assay has been developed for high-dose estimations, but little attention has been given to its applicability for partial-body irradiations. The estimation of the dose and the irradiated fraction of the body is important information in the primary medical response in case of a radiological accident. it allows fully automated mitotic and nuclei scoring in cytogenetic images yielding reliable information with minimal user intervention. The CellProfiler automated pipeline achieves good agreement with visual counting workflow, i.e.
Cellprofiler metadata manual#
We conclude that CellProfiler is a reliable tool for counting mitosis and nuclei from cytogenetic images, saves considerable time compared to manual operation and reduces the variability derived from the scoring criteria of different scorers. The CellProfiler pipeline includes the detection of nuclei and mitosis with 80% sensitivity and more than 99% specificity. Sets of images from cultures were analyzed visually and findings were compared with those using CellProfiler software. Lymphocytes were irradiated and cultured for mitosis detection.
Cellprofiler metadata software#
The objective of this paper was to apply the CellProfiler open-source software for automatic detection of mitotic and nuclei figures from digitized images of cultured human lymphocytes for MI assessment, and to compare its performance to that performed through semi-automatic and visual detection. To this end, MI is applied to check the lot of media and reagents to be used throughout the assay and also to check cellular viability after blood sample shipping, indicating satisfactory/unsatisfactory conditions for the progression of cell culture. Mitotic Index (MI) estimation expressed as percentage of mitosis plays an important role as quality control endpoint. Conclusions: The results obtained indicate that, despite the problems identified in few partners, which can be corrected, the RENEB consortium is able to carry out retrospective biodosimetry analyzing the frequency of translocations by FISH.
![cellprofiler metadata cellprofiler metadata](https://training.galaxyproject.org/archive/2022-08-01/topics/imaging/images/tutorial-CP/img_dna_channel.png)
Z-score analysis revealed that eight out of 10 and 17 out of 20 dose estimates were satisfactory in the first and second IE, respectively.
![cellprofiler metadata cellprofiler metadata](https://cellprofiler.org/files/cellprofiler/files/specklescd_1.gif)
Results: The coefficient of variation in the first IE (CV = 0.34) was higher than in the second IE (CV = 0.16 and 0.23 in the two samples analyzed), for the genomic frequency of total translocations. Samples were cultured and stained by each partner using their standard protocol and translocation frequency was used to produce dose estimates. Then two blind intercomparison exercises (IE) were performed, by sending irradiated blood samples to each partner. Materials and methods: An initial tele-scoring exercise analyzing FISH metaphase images was done to harmonize chromosome aberration descriptions. The aim of this study was to harmonize the accuracy of translocation-based biodosimetry within the RENEB consortium. Among them, the analysis of translocations is considered the most useful method for retrospective biodosimetry due to the relative stability of their frequency with post irradiation time. Purpose: In the framework of RENEB, several biodosimetry exercises were conducted analyzing different endpoints.
![Human japanese intermediate crack](https://loka.nahovitsyn.com/76.jpg)